e hormaechei subsp oharae dsm 16687  (ATCC)

Journal: Microorganisms

Article Title: Presence and Role of the Type 3 Fimbria in the Adherence Capacity of Enterobacter hormaechei subsp. hoffmannii

doi: 10.3390/microorganisms12071441

Figure Lengend Snippet: Strains and plasmids used in this work.

Article Snippet: Subspecies were identified by performing a phylogenetic analysis with kSNP 3.1 [ ], including genomes of strains E. hormaechei subsp. hoffmannii DSM 14563, E. hormaechei subsp. hormaechei ATCC 49162, E. hormaechei subsp. oharae DSM 16687, E. hormaechei subsp. steigerwaltii DSM 16691, and E. hormaechei subsp. xiangfangensis LMG 27195 as controls ( ) [ ].

Techniques: Functional Assay, Transformation Assay, Plasmid Preparation, Expressing

Journal: Microorganisms

Article Title: Presence and Role of the Type 3 Fimbria in the Adherence Capacity of Enterobacter hormaechei subsp. hoffmannii

doi: 10.3390/microorganisms12071441

Figure Lengend Snippet: Presence of the type 3 fimbriae among Enterobacter cloacae complex strains and E. hormaechei subsp. hoffmannii strains: ( A ) Antibiotic susceptibility profile of eight E. cloacae complex strains. S: susceptible, R: resistant. Trimethropim-SMX: trimethoprim–sulfamethoxazole. ( B ) The type 3 fimbria major structural subunit MrkA was detected by Western blot in heat-extracted proteins. The purified mature MrkA protein and extracts obtained from the Klebsiella pneumoniae strain Kpn1-UCH were used as positive controls. ( C ) Maximum parsimony phylogenetic tree to identify E. hormaechei subspecies. Genomes of strains Eh12-UCH, Eh13-UCH, Eh18-UCH, and Eh31-UCH were included along with genomes of the type strains E. hormaechei subsp. hoffmannii DSM 14563, E. hormaechei subsp. hormaechei ATCC 49162, E. hormaechei subsp. oharae DSM 16687, E. hormaechei subsp. steigerwaltii DSM 16691, and E. hormaechei subsp. xiangfangensis LMG 27195 . The tree was built based on 48,321 core SNPs. ( D ) Identification of E. hormaechei subspecies by average nucleotide identity (ANI) analysis. The heat map represents the results for the same set of genomes included in ( C ). ( E ) Detection of MrkA by Western blot in heat-extracted proteins obtained from the mutant strain Eh13-UCHΔ mrkA and its derivative complemented strains.

Article Snippet: Subspecies were identified by performing a phylogenetic analysis with kSNP 3.1 [ ], including genomes of strains E. hormaechei subsp. hoffmannii DSM 14563, E. hormaechei subsp. hormaechei ATCC 49162, E. hormaechei subsp. oharae DSM 16687, E. hormaechei subsp. steigerwaltii DSM 16691, and E. hormaechei subsp. xiangfangensis LMG 27195 as controls ( ) [ ].

Techniques: Western Blot, Purification, Mutagenesis

Journal: Microorganisms

Article Title: Presence and Role of the Type 3 Fimbria in the Adherence Capacity of Enterobacter hormaechei subsp. hoffmannii

doi: 10.3390/microorganisms12071441

Figure Lengend Snippet: Distribution of the type 3 pili genes among E. hormaechei genomes contained in the NCBI Assembly RefSeq database: ( A ) Screening for the mrkABCDF locus and its individual genes using blastn. The graph shows the blast-score ratio (BSR) values. ( B , C ) Distribution of BSR values in the screening of individual genes among records that showed BSR equal to or higher than 0.9 for the screening of the mrkABCDF locus, using blastn ( B ) or tblastn ( C ). Horizontal dotted lines in ( A – C ) represent the means. ( D ) Identification of subspecies among positive records selected according to two criteria, BSR ≥ 0.9 for the mrkABCDF locus with blastn or BSR ≥ 0.9 for mrkC with tblastn. Numbers above the bars indicate the percentage of positive records among genomes representing each subspecies. Total numbers (100%) were 714 E. hormaechei subsp. hoffmannii, 47 E. hormaechei subsp. hormaechei, 165 E. hormaechei subsp. oharae, 1257 E. hormaechei subsp. steigerwaltii , and 1032 E. hormaechei subsp. xiangfangensis. ( E ) Localization of the mrkABCDF locus, analyzed in 23 complete genomes. No complete genomes representing E. hormaechei subsp. hormaechei were found. ( F , G ) Distribution of sequence type among E. hormaechei genome records positive for type 3 fimbriae, selected according to both criteria, BSR ≥ 0.9 in the screening for the mrkABCDF locus using blastn ( F ) or BSR ≥ 0.9 in the screening for mrkC with tblastn ( G ).

Article Snippet: Subspecies were identified by performing a phylogenetic analysis with kSNP 3.1 [ ], including genomes of strains E. hormaechei subsp. hoffmannii DSM 14563, E. hormaechei subsp. hormaechei ATCC 49162, E. hormaechei subsp. oharae DSM 16687, E. hormaechei subsp. steigerwaltii DSM 16691, and E. hormaechei subsp. xiangfangensis LMG 27195 as controls ( ) [ ].

Techniques: Sequencing

Journal: Antimicrobial Agents and Chemotherapy

Article Title: MgrB Inactivation Is Responsible for Acquired Resistance to Colistin in Enterobacter hormaechei subsp. steigerwaltii

doi: 10.1128/AAC.00128-20

Figure Lengend Snippet: Colistin MIC of strains and transformants of E. hormaec hei and K. pneumoniae

Article Snippet: In E. hormaechei metacluster (A to E groups): A, E. xiangfangensis LMG 27195 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017183","term_id":"1068585475","term_text":"NZ_CP017183"}} NZ_CP017183 ; B, E. hormaechei subsp. steigerwaltii DSM 16691 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017179","term_id":"1068564097","term_text":"NZ_CP017179"}} NZ_CP017179 ; C, E. hormaechei subsp. oharae DSM 16687 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017180","term_id":"1068589113","term_text":"NZ_CP017180"}} NZ_CP017180 ; D, E. hormaechei subsp. hoffmannii DSM 14563 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017186","term_id":"1068570424","term_text":"NZ_CP017186"}} NZ_CP017186 ; E, E. hormaechei ATCC 49162, {"type":"entrez-nucleotide","attrs":{"text":"AFHR01000000","term_id":"333392840","term_text":"gb||AFHR01000000"}} AFHR01000000 .

Techniques: Plasmid Preparation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: MgrB Inactivation Is Responsible for Acquired Resistance to Colistin in Enterobacter hormaechei subsp. steigerwaltii

doi: 10.1128/AAC.00128-20

Figure Lengend Snippet: MgrB alignment comparison of Eh22. (A) MgrB alignments of Eh22 with peptidic sequences from 15 of 18 Enterobacter cloacae complex groups as defined by Chavda et al. (3). MgrB sequences used for alignments were selected as follows: group, name of species, name of strain (strain type, when available, is designated by the superscript T), and GenBank accession number. In E. hormaechei metacluster (A to E groups): A, E. xiangfangensis LMG 27195T, NZ_CP017183; B, E. hormaechei subsp. steigerwaltii DSM 16691T, NZ_CP017179; C, E. hormaechei subsp. oharae DSM 16687T, NZ_CP017180; D, E. hormaechei subsp. hoffmannii DSM 14563T, NZ_CP017186; E, E. hormaechei ATCC 49162, AFHR01000000. In the E. cloacae metacluster (G to N, P and Q groups): G, E. cloacae subsp. cloacae ATCC 13047, NC_014121; H, E. cloacae subsp. dissolvens SDM, NC_018079; I, E. ludwigii EN-119, CP017279; J, E. asburiae ATCC 35953, CP011863; K, E. cloacae complex DC4, AZUB01000000; L, E. cloacae complex BWH 43, JMUR01000000; M, E. cloacae complex DSM 16690T, CP017184.1; N, E. cloacae complex SY-70, NZ_JALR01000000; P, E. cloacae complex JD8715, JDWG01000000; Q, E. kobei DSM 13645T, CP017181. In E. hormaechei metacluster, three groups have unavailable MgrB sequences: F (E. mori), O, and R (E. cloacae complex). (B) MgrB alignments of E. coli K-12, Eh22, and K. pneumoniae Kp5196. The putative transmembrane domain of E. coli MgrB is underlined according to Lippa and Goulian (29), and the amino acid at position 10 is indicated in bold. The MgrB sequences of Eh22 (A) and E. coli K-12 (B) are given, and only different residues are shown for the other alignments.

Article Snippet: In E. hormaechei metacluster (A to E groups): A, E. xiangfangensis LMG 27195 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017183","term_id":"1068585475","term_text":"NZ_CP017183"}} NZ_CP017183 ; B, E. hormaechei subsp. steigerwaltii DSM 16691 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017179","term_id":"1068564097","term_text":"NZ_CP017179"}} NZ_CP017179 ; C, E. hormaechei subsp. oharae DSM 16687 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017180","term_id":"1068589113","term_text":"NZ_CP017180"}} NZ_CP017180 ; D, E. hormaechei subsp. hoffmannii DSM 14563 T , {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP017186","term_id":"1068570424","term_text":"NZ_CP017186"}} NZ_CP017186 ; E, E. hormaechei ATCC 49162, {"type":"entrez-nucleotide","attrs":{"text":"AFHR01000000","term_id":"333392840","term_text":"gb||AFHR01000000"}} AFHR01000000 .

Techniques: Comparison